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hfl 1 cells  (ATCC)


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    ATCC hfl 1 cells
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Hfl 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfl 1 cells/product/ATCC
    Average 96 stars, based on 630 article reviews
    hfl 1 cells - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary Fibrosis"

    Article Title: Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary Fibrosis

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms27094128

    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Figure Legend Snippet: Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

    Techniques Used: Activity Assay, Co-Culture Assay, Control, Fluorescence, Imaging, Labeling, Quantitative RT-PCR, Expressing



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    Image Search Results


    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

    Journal: International Journal of Molecular Sciences

    Article Title: Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary Fibrosis

    doi: 10.3390/ijms27094128

    Figure Lengend Snippet: Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

    Article Snippet: Phoenix-AMPHO and HFL-1 cells were obtained from the American Type Culture Collection (ATCC).

    Techniques: Activity Assay, Co-Culture Assay, Control, Fluorescence, Imaging, Labeling, Quantitative RT-PCR, Expressing